10.6084/m9.figshare.1547921.v2 Dr. Cristina Suarez Dr. Cristina Suarez Dr. German Andres Dr. German Andres Mrs. Androniki Kolovou Mrs. Androniki Kolovou Mrs. Simone Hoppe Mrs. Simone Hoppe Prof. Maria Luisa Salas Prof. Maria Luisa Salas Dr. Paul Walther Dr. Paul Walther Prof. Jacomine Krijnse Locker Prof. Jacomine Krijnse Locker Data from: AFRICAN SWINE FEVER VIRUS ASSEMBLES A SINGLE MEMBRANE DERIVED FROM RUPTURE OF THE ENDOPLASMIC RETICULUM Wiley 2016 membrane virus electron microscopy tomography asfv Cell Biology Microbiology 2016-07-14 17:14:30 Dataset https://wiley.figshare.com/articles/dataset/AFRICAN_SWINE_FEVER_VIRUS_ASSEMBLES_A_SINGLE_MEMBRANE_DERIVED_FROM_RUPTURE_OF_THE_ENDOPLASMIC_RETICULUM/1547921 <p>Collective evidence argues that two members of the Nucleo-cytoplasmic large DNA viruses (NCLDVs) acquire their membrane from open membrane intermediates, postulated to be derived from membrane rupture. We now study membrane acquisition of the NCLDV African swine fever virus (ASFV). By electron tomography (ET) the virion assembles a single bilayer derived open membrane precursors that collect as ribbons in the cytoplasm. Biochemically, lumenal ER proteins are released into the cytosol, arguing that the open intermediates are ruptured ER membranes. ET shows that viral capsid assembles on the convex side of the open viral membrane to shape it into an icosahedron. The viral capsid is composed of tiny spikes with a diameter of ~5nm, connected to the membrane by a 6nm wide structure displaying thin striations, as assessed by several complementary EM imaging methods. Immature particles display an opening that closes after uptake of the viral genome and core proteins, followed by the formation of the mature virion. Together with our previous data this study shows a common principle of NCLDVs to build a single internal envelope from open membrane intermediates. Our data now provide biochemical evidence that these open intermediates result from rupture of a cellular membrane, the ER.</p>