Dataset for: The regulatory significance of tag recycling in the mycobacterial Pup-proteasome system Yifat Elharar Shai Schlussel Nir Hecht Michael M. Meijler Eyal Gur 10.6084/m9.figshare.5001905.v1 https://wiley.figshare.com/articles/dataset/Dataset_for_The_regulatory_significance_of_tag_recycling_in_the_mycobacterial_Pup-proteasome_system/5001905 Pup, a ubiquitin analog, tags proteins for degradation by the bacterial proteasome. As an intracellular proteolytic system, the Pup-proteasome system (PPS) must be carefully regulated to prevent excessive protein degradation. Currently, those factors underlying PPS regulation remain poorly understood. Here, experimental analysis combined with theoretical modeling of in vivo protein pupylation revealed how the basic PPS design allows stable and controlled protein pupylation. Specifically, the recycling of Pup when targets are degraded allows the PPS to maintain steady state levels of protein pupylation and degradation at a rate limited by proteasome function, and at a pupylome level limited by Pup concentrations. This design allows the Pup-ligase, a highly promiscuous enzyme, to act in a controlled manner without causing damage, and the PPS to be effectively tuned to control protein degradation. This study thus provides understanding of how the inherent design of an intracellular proteolytic system serves crucial regulatory purposes. 2017-05-12 08:45:02 proteolysis Dop Pup PafA proteasome Stem Cells Biochemistry Molecular Biology Developmental Biology Structural Biology Synthetic Biology Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Evolutionary Biology Signal Transduction Cancer Cell Biology Systems Biology Bioinformatics Heat and Mass Transfer Operations