Dataset for: The regulatory significance of tag recycling in the mycobacterial Pup-proteasome system
Yifat Elharar
Shai Schlussel
Nir Hecht
Michael M. Meijler
Eyal Gur
10.6084/m9.figshare.5001905.v1
https://wiley.figshare.com/articles/dataset/Dataset_for_The_regulatory_significance_of_tag_recycling_in_the_mycobacterial_Pup-proteasome_system/5001905
Pup, a ubiquitin analog, tags proteins for degradation by the bacterial proteasome. As an intracellular proteolytic system, the Pup-proteasome system (PPS) must be carefully regulated to prevent excessive protein degradation. Currently, those factors underlying PPS regulation remain poorly understood. Here, experimental analysis combined with theoretical modeling of in vivo protein pupylation revealed how the basic PPS design allows stable and controlled protein pupylation. Specifically, the recycling of Pup when targets are degraded allows the PPS to maintain steady state levels of protein pupylation and degradation at a rate limited by proteasome function, and at a pupylome level limited by Pup concentrations. This design allows the Pup-ligase, a highly promiscuous enzyme, to act in a controlled manner without causing damage, and the PPS to be effectively tuned to control protein degradation. This study thus provides understanding of how the inherent design of an intracellular proteolytic system serves crucial regulatory purposes.
2017-05-12 08:45:02
proteolysis
Dop
Pup
PafA
proteasome
Stem Cells
Biochemistry
Molecular Biology
Developmental Biology
Structural Biology
Synthetic Biology
Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Evolutionary Biology
Signal Transduction
Cancer Cell Biology
Systems Biology
Bioinformatics
Heat and Mass Transfer Operations