10.6084/m9.figshare.5271613.v1 Alfonso Galan Alfonso Galan Gloria Lozano Gloria Lozano David Piñeiro David Piñeiro Encarnación (Encarna) Martínez-Salas Encarnación Martínez-Salas Dataset for: G3BP1 interacts directly with the FMDV IRES and negatively regulates translation Wiley 2017 G3BP1-RNA interaction translation control IRES elements FMDV RNA structure Stem Cells Biochemistry Molecular Biology Developmental Biology Structural Biology Synthetic Biology Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Evolutionary Biology Signal Transduction Cancer Cell Biology Systems Biology Bioinformatics Heat and Mass Transfer Operations 2017-09-01 07:22:00 Dataset https://wiley.figshare.com/articles/dataset/Dataset_for_G3BP1_interacts_directly_with_the_FMDV_IRES_and_negatively_regulates_translation/5271613 RNA-protein interactions play a pivotal role in the function of picornavirus internal ribosome entry site (IRES) elements. Here we analysed the impact of Ras GTPase SH3 domain binding protein 1 (G3BP1) in the IRES activity of foot-and-mouth disease virus (FMDV). We found that G3BP1 interacts directly with three distinct sequences of the IRES element using RNA electrophoretic mobility-shift assays. Analysis of the interaction with domain 5 indicated that the G3BP1 binding-site is placed at the single-stranded region although it allows large sequence heterogeneity and the hairpin located upstream of this region enhances retarded complex formation. In addition, G3BP1 interacts directly with the polypyrimidine tract-binding protein (PTB) and the translation initiation factor 4B (eIF4B) through the C-terminal region. Moreover, G3BP1 is cleaved during FMDV infection yielding two fragments, Ct-G3BP1 and Nt-G3BP1. Both fragments inhibit cap- and IRES-dependent translation, but the Ct-G3BP1 fragment shows a stronger effect on IRES-dependent translation. Assembly of complexes with G3BP1 results in a significantly reduced local flexibility of the IRES element, consistent with the negative effect of this protein. Our results highlight the IRES-binding capacity of G3BP1 and illustrate its function as a translation inhibitor.