Dataset for: G3BP1 interacts directly with the FMDV IRES and negatively regulates translation
Alfonso Galan
Gloria Lozano
David Piñeiro
Encarnación (Encarna) Martínez-Salas
10.6084/m9.figshare.5271613.v1
https://wiley.figshare.com/articles/dataset/Dataset_for_G3BP1_interacts_directly_with_the_FMDV_IRES_and_negatively_regulates_translation/5271613
RNA-protein interactions play a pivotal role in the function of picornavirus internal ribosome entry site (IRES) elements. Here we analysed the impact of Ras GTPase SH3 domain binding protein 1 (G3BP1) in the IRES activity of foot-and-mouth disease virus (FMDV). We found that G3BP1 interacts directly with three distinct sequences of the IRES element using RNA electrophoretic mobility-shift assays. Analysis of the interaction with domain 5 indicated that the G3BP1 binding-site is placed at the single-stranded region although it allows large sequence heterogeneity and the hairpin located upstream of this region enhances retarded complex formation. In addition, G3BP1 interacts directly with the polypyrimidine tract-binding protein (PTB) and the translation initiation factor 4B (eIF4B) through the C-terminal region. Moreover, G3BP1 is cleaved during FMDV infection yielding two fragments, Ct-G3BP1 and Nt-G3BP1. Both fragments inhibit cap- and IRES-dependent translation, but the Ct-G3BP1 fragment shows a stronger effect on IRES-dependent translation. Assembly of complexes with G3BP1 results in a significantly reduced local flexibility of the IRES element, consistent with the negative effect of this protein. Our results highlight the IRES-binding capacity of G3BP1 and illustrate its function as a translation inhibitor.
2017-09-01 07:22:00
G3BP1-RNA interaction
translation control
IRES elements
FMDV
RNA structure
Stem Cells
Biochemistry
Molecular Biology
Developmental Biology
Structural Biology
Synthetic Biology
Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Evolutionary Biology
Signal Transduction
Cancer Cell Biology
Systems Biology
Bioinformatics
Heat and Mass Transfer Operations