Dataset for: RNA editing enzymes ADAR1 and ADAR2 coordinately regulate the editing and expression of Ctn RNA
Aparna Anantharaman
Omid Gholamalamdari
Abid Khan
Je-Hyun Yoon
Michael Jantsch
Jochen C Hartner
Myriam Gorospe
Supriya G. Prasanth
KV Prasanth
10.6084/m9.figshare.5306737.v1
https://wiley.figshare.com/articles/dataset/Dataset_for_RNA_editing_enzymes_ADAR1_and_ADAR2_coordinately_regulate_the_editing_and_expression_of_Ctn_RNA/5306737
Adenosine Deaminases acting on RNA (ADARs) are proteins that catalyze widespread A-to-I editing within RNA sequences. We recently reported that ADAR2 edits and stabilizes nuclear-retained Cat2 transcribed nuclear RNA (Ctn RNA). Here, we report that ADAR1 coordinates with ADAR2 to regulate editing and stability of Ctn RNA. We observe an RNA-dependent interaction between ADAR1 and ADAR2. Furthermore, ADAR1 negatively regulates interaction of Ctn RNA with RNA-destabilizing proteins. We also show that breast cancer (BC) cells display elevated ADAR1 but not ADAR2 levels, compared to non-tumorigenic cells. Additionally, BC patients with elevated levels of ADAR1 show low survival. Our findings provide insights into overlapping substrate preferences of ADARs and potential involvement of ADAR1 in breast cancer.
2017-08-30 13:17:00
ADAR; Ctn RNA; RNA editing; mRNA stability; breast cancer; HuR
Biophysics
Synthetic Biology
Biochemistry
Plant Biology
Virology
Receptors and Membrane Biology
Computational Biology
Immunology
Neuroscience
Cell Development, Proliferation and Death
Molecular Biology
Evolutionary Biology
Signal Transduction
Cancer Cell Biology
Systems Biology
Structural Biology