Dataset for: Novel landscape of HLA-G isoforms in clear cell renal cell carcinoma patients Diana Tronik-Le Roux Julie Renard Jerome Verine Victor Renault Emmanuel Tubacher Joël LeMaoult Nathalie Rouas-Freiss Jean-François Deleuze François Desgrandchamps Edgardo D Carosella 10.6084/m9.figshare.5319364.v1 https://wiley.figshare.com/articles/dataset/Dataset_for_Novel_landscape_of_HLA-G_isoforms_in_clear_cell_renal_cell_carcinoma_patients/5319364 Immune-checkpoints are powerful inhibitory molecules that promote tumor survival. Their blockade is now recognized as providing effective therapeutic benefit against cancer. HLA-G, a recently identified immune checkpoint, has been detected in many types of primary tumors and metastases, in malignant effusions as well as on tumor-infiltrating cells, particularly in patients with clear cell renal cell carcinoma (ccRCC). Here, in order to define a possible anti-cancer therapy, we used a molecular approach based on an unbiased strategy that combines transcriptome determination and immunohistochemical labeling, to analyze in-depth, the HLA-G isoforms expressed in these tumors. We found that the expression of HLA-G is highly variable among tumors and distinct areas of the same tumor, testifying a marked inter- and intra-tumor heterogeneity. Moreover, our results generate an inventory of novel HLA-G isoforms which includes spliced forms that have an extended 5’-region and lack the transmembrane and alpha-1 domains. So far, these isoforms could not be detected by any method available and their assessment may improve the procedure by which tumors are analyzed. Collectively, our approach provides the first extensive portrait of HLA-G in ccRCC and reveals data that should prove suitable for the tailoring of future clinical applications. 2017-09-13 12:07:00 HLA-G Transcriptome Immune checkpoints RNAseq Isoforms ccRCC Cancer Tumour Immunology Medical Biochemistry and Metabolomics not elsewhere classified Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Molecular Biology Clinical Pharmacology and Therapeutics Biomarkers Genomics Cancer Cell Biology Cancer Genetics Chemotherapy