Dataset for: MicroRNA-661 modulates redox and metabolic homeostasis in Colon Cancer
Marta Gómez de Cedrón
Rebeca Acin
Ruth Sanchez-Martinez
Susana Molina
Jesús Herranz
Jaime Feliu
Guillermo Reglero
Jose-Antonio Enriquez
Ana Ramírez de Molina
10.6084/m9.figshare.5484376.v1
https://wiley.figshare.com/articles/dataset/Dataset_for_MicroRNA-661_modulates_redox_and_metabolic_homeostasis_in_Colon_Cancer/5484376
Cancer cell survival and metastasis are dependent on a metabolic reprogramming able to increase resistance to oxidative and energetic stress. Targeting these two processes can be crucial for cancer progression. Herein we describe the role of microRNA-661 (miR661) as epigenetic regulator of colon cancer (CC) cell metabolism. miR661 induces a global increase in reactive oxygen species (ROS), specifically in mitochondrial superoxide anions (SO-), that seems to be mediated by decreased carbohydrate metabolism and pentose phosphate pathway, and by a higher dependency on mitochondrial respiration. miR661 overexpression in non-metastatic human CC cells induces an epithelial to mesenchymal transition (EMT) phenotype and a reduced tolerance to metabolic stress. This seems to be a general effect of miR661 in CC, since metastatic CC cells metabolism is also compromised upon miR661 overexpression. We propose hexose 6 phosphate dehydrogenase (H6PD) and pyruvate kinase M2 (PKM2) as two players related to the observed metabolic reprogramming. Finally, the clinical relevance of miR661 expression levels in stage-II and III CC patients is discussed. In conclusion, we propose miR661 as a potential modulator of redox and metabolic homeostasis in CC.
2017-10-11 13:02:09
miR
oxidative stress
metabolomics
bioenergetics
colon cancer
Cancer
Tumour Immunology
Medical Biochemistry and Metabolomics not elsewhere classified
Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Molecular Biology
Clinical Pharmacology and Therapeutics
Biomarkers
Genomics
Cancer Cell Biology
Cancer Genetics
Chemotherapy