10.6084/m9.figshare.6507029.v1 Morium Begam Morium Begam Alyssa F Collier Alyssa F Collier Amber L Mueller Amber L Mueller Renuka Roche Renuka Roche Sujay S Galen Sujay S Galen Joseph A Roche Joseph A Roche Dataset for: Diltiazem improves contractile properties of skeletal muscle in dysferlin-deficient BLAJ mice, but does not reduce contraction-induced muscle damage Wiley 2018 Skeletal muscle dysferlin LGMD2B Miyoshi myopathy diltiazem muscle damage eccentric contractions Physiology Systems Biology 2018-08-07 06:29:09 Dataset https://wiley.figshare.com/articles/dataset/Dataset_for_Diltiazem_improves_contractile_properties_of_skeletal_muscle_in_dysferlin-deficient_BLAJ_mice_but_does_not_reduce_contraction-induced_muscle_damage/6507029 B6.A-<i>Dysf</i><i><sup>prmd</sup></i>/GeneJ (BLAJ) mice model human limb-girdle muscular dystrophy 2B (LGMD2B), which is linked to mutations in the dysferlin (DYSF) gene. We tested the hypothesis that, the calcium ion (Ca2+) channel blocker diltiazem (DTZ), reduces contraction-induced skeletal muscle damage, in BLAJ mice. We randomly assigned mice (N = 12; 3 - 4 m old males) to one of two groups – DTZ (N = 6) or vehicle (VEH, distilled water, N = 6). We conditioned mice with either DTZ or VEH for 1 wk, after which, their tibialis anterior (TA) muscles were tested for contractile torque and susceptibility to injury from forced eccentric contractions. We continued dosing with DTZ or VEH for 3 days following eccentric contractions, and then studied torque recovery and muscle damage. We analyzed contractile torque before eccentric contractions, immediately after eccentric contractions, and at 3 days after eccentric contractions; and counted damaged fibers in the injured and uninjured TA muscles. We found that DTZ improved contractile torque before and immediately after forced eccentric contractions, but did not reduce delayed-onset muscle damage that was observed at 3 days after eccentric contractions.