%0 Generic
%A Begam, Morium
%A Collier, Alyssa F
%A Mueller, Amber L
%A Roche, Renuka
%A Galen, Sujay S
%A Roche, Joseph A
%D 2018
%T Dataset for: Diltiazem improves contractile properties of skeletal muscle in dysferlin-deficient BLAJ mice, but does not reduce contraction-induced muscle damage
%U https://wiley.figshare.com/articles/dataset/Dataset_for_Diltiazem_improves_contractile_properties_of_skeletal_muscle_in_dysferlin-deficient_BLAJ_mice_but_does_not_reduce_contraction-induced_muscle_damage/6507029
%R 10.6084/m9.figshare.6507029.v1
%2 https://wiley.figshare.com/ndownloader/files/11970161
%K Skeletal muscle
%K dysferlin
%K LGMD2B
%K Miyoshi myopathy
%K diltiazem
%K muscle damage
%K eccentric contractions
%K Physiology
%K Systems Biology
%X B6.A-Dysfprmd/GeneJ (BLAJ) mice model human limb-girdle muscular dystrophy 2B (LGMD2B), which is linked to mutations in the dysferlin (DYSF) gene. We tested the hypothesis that, the calcium ion (Ca2+) channel blocker diltiazem (DTZ), reduces contraction-induced skeletal muscle damage, in BLAJ mice. We randomly assigned mice (N = 12; 3 - 4 m old males) to one of two groups – DTZ (N = 6) or vehicle (VEH, distilled water, N = 6). We conditioned mice with either DTZ or VEH for 1 wk, after which, their tibialis anterior (TA) muscles were tested for contractile torque and susceptibility to injury from forced eccentric contractions. We continued dosing with DTZ or VEH for 3 days following eccentric contractions, and then studied torque recovery and muscle damage. We analyzed contractile torque before eccentric contractions, immediately after eccentric contractions, and at 3 days after eccentric contractions; and counted damaged fibers in the injured and uninjured TA muscles. We found that DTZ improved contractile torque before and immediately after forced eccentric contractions, but did not reduce delayed-onset muscle damage that was observed at 3 days after eccentric contractions.
%I Wiley