Dataset for: Model discrimination for Ca<sup>2+</sup>-dependent regulation of myosin light chain kinase in smooth muscle contraction Joseph P. Dexter John W. Biddle Jeremy Gunawardena 10.6084/m9.figshare.6871136.v1 https://wiley.figshare.com/articles/dataset/Dataset_for_Model_discrimination_for_Ca_sup_2_sup_-dependent_regulation_of_myosin_light_chain_kinase_in_smooth_muscle_contraction/6871136 Excitation-contraction coupling in smooth muscle is mediated by the Ca<sup>2+</sup>- and calmodulin-dependent regulation of myosin light chain kinase. The precise mechanism of this regulation remains controversial, and several mathematical models have been proposed for the interaction of the three species. These models have previously been analyzed at steady state primarily by numerical simulation of differential equations, for which parameter values must be estimated from data. Here, we use the linear framework for timescale separation to demonstrate that models of this general kind can be solved analytically for an equilibrium steady state, without having to determine parameter values. This analysis leads to parameter-independent methods for discriminating between the models, for which we propose experiments that could be performed with existing methods. 2018-09-06 11:33:10 calcium signaling linear framework mathematical modeling Biophysics Synthetic Biology Biochemistry Plant Biology Virology Receptors and Membrane Biology Computational Biology Immunology Neuroscience Cell Development, Proliferation and Death Molecular Biology Evolutionary Biology Signal Transduction Cancer Cell Biology Systems Biology Structural Biology