10.6084/m9.figshare.7001162.v1
Matthew Schwede
Matthew
Schwede
Erin Wilfong
Erin
Wilfong
Rachel L Zemans
Rachel
L Zemans
Patty J Lee
Patty J
Lee
Claudia dos Santos
Claudia dos
Santos
Xiaohui Fang
Xiaohui
Fang
Michael A Matthay
Michael
A Matthay
Dataset for: Effects of Bone Marrow-Derived Mesenchymal Stromal Cells on Gene Expression in Human Alveolar Type II Cells Exposed to TNF-α, IL-1β, and IFN-γ
Wiley
2018
Acute respiratory distress syndrome
Alveolar epithelial cells
Mesenchymal stromal cells
Gene expression profiling
Physiology
Systems Biology
2018-08-23 14:20:18
Dataset
https://wiley.figshare.com/articles/dataset/Dataset_for_Effects_of_Bone_Marrow-Derived_Mesenchymal_Stromal_Cells_on_Gene_Expression_in_Human_Alveolar_Type_II_Cells_Exposed_to_TNF-_IL-1_and_IFN-_/7001162
The acute respiratory distress syndrome (ARDS) is common in critically ill patients and has a high mortality rate. Mesenchymal stromal cells (MSCs) have demonstrated therapeutic potential in animal models of ARDS, and their benefits occur in part through interactions with alveolar type II (ATII) cells. However, the effects that MSCs have on human ATII cells have not been well studied. Using previously published microarray data, we performed genome-wide differential gene expression analyses of human ATII cells that were 1) unstimulated, 2) exposed to proinflammatory cytokines (CytoMix), or 3) exposed to proinflammatory cytokines plus MSCs. Findings were validated by qPCR. Alveolar type II cells differentially expressed hundreds of genes when exposed either to proinflammatory cytokines or proinflammatory cytokines plus MSCs. Stimulation with proinflammatory cytokines increased expression of inflammatory genes and down-regulated genes related to surfactant function and alveolar fluid clearance. Some of these changes, including expression of some cytokines and genes related to surfactant, were reversed by exposure to MSCs. In addition, MSCs induced up-regulation of other potentially beneficial genes, such as those related to extracellular matrix remodeling. We confirmed several of these gene expression changes by qPCR. Thus, ATII cells down-regulate genes associated with surfactant and alveolar fluid clearance when exposed to inflammatory cytokines, and mesenchymal stromal cells partially reverse many of these gene expression changes.