Dataset for: mRNA 5’ ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced Mikaela R Berger Rolando Alvarado Daniel Louis Kiss 10.6084/m9.figshare.7764359.v1 https://wiley.figshare.com/articles/dataset/Dataset_for_mRNA_5_ends_targeted_by_cytoplasmic_recapping_cluster_at_CAGE_tags_and_select_transcripts_are_alternatively_spliced/7764359 Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5’ RACE shows that nearly all uncapped ends correspond to CAGE tags and that the recapping of <i>ZNF207 </i>mRNA may be restricted to a single splice isoform. A modified RACE approach detected uncapped 5’ RNA ends mapping to 46 mRNAs in dominant negative cytoplasmic capping enzyme expressing and normal cells. 11 of 46 cloned mRNAs also contained splice isoform-limiting sequences. Collectively, these data reinforce earlier work and suggest that alternative splicing may play a role in targeting transcripts for– and/or determining the position of– cytoplasmic capping. 2019-08-01 14:03:14 cytoplasmic recapping CAGE cap homeostasis alternative splicing stable uncapped 5’ ends epitranscriptomics Biophysics Synthetic Biology Biochemistry Plant Biology Virology Cell Development, Proliferation and Death Computational Biology Immunology Neuroscience Receptors and Membrane Biology Molecular Biology Evolutionary Biology Signal Transduction Cancer Cell Biology Systems Biology Structural Biology