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Dataset for: Baicalin targets the AKT-PH domain, activates AKT/GSK3β phosphorylation and improves glucose metabolism
dataset
posted on 2018-12-17, 09:33 authored by Shengnan Yang, Yuan Zhang, Fukuai Shen, Xiaoyao Ma, Man Zhang, Yuanyuan Hou, Gang BaiBaicalin is one of the main flavonoids of the dried root of Scutellaria baicalensis Georgi and is reported to exert beneficial effects on the regulation of glucose/lipid metabolism. However, understanding the specific target and unique mechanism of improving glucose utilization is a challenge. In this paper, target fishing via with baicalin probe revealed that baicalin was bound with AKT. An immunofluorescence assay further demonstrated the colocalization of baicalin with AKT in the cytoplasm. A competitive test and virtual docking showed that baicalin might bind to the pleckstrin homology (PH) domain of AKT. The specific binding hampered AKT membrane translocation, activated the phosphorylation of AKT on Ser473, induced the downstream glycogen synthase kinase 3β (GSK3β) activation, and affected glycogen synthesis.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Receptors and membrane biology
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Cell development, proliferation and death
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
baicalinglucose metabolismAKTPH domainGSK3βBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyReceptors and Membrane BiologyComputational BiologyImmunologyNeuroscienceCell Development, Proliferation and DeathMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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