Supplementary-Data_Peptide-List.xlsx (92.82 kB)
Dataset for: Characterization of a Polypeptide Binding Site in the DEAD Motor of the SecA ATPase
dataset
posted on 2017-09-12, 05:02 authored by Aliakbar Khalili-Yazdi, Sarita Namjoshi, Jesse Hackett, Nour Ghonaim, Brian H. ShiltonPeptides from a CNBr digest of signal-sequenceless maltose binding protein (MBP) were coupled to a surface plasmon resonance (SPR) chip. SecA-N95, SecA-N68, and SecA-DM (which consists of only the DEAD Motor domains, NBD1 and NBD2) bound to the immobilized peptides; ADP weakened the binding. SecA-DM, which lacks the “preprotein cross-linking domain” (PPXD), displayed the most extensive binding, while an MBP-PPXD chimera showed no binding, demonstrating that the PPXD does not contribute to the binding. The sequence specificity was characterized using oriented peptide libraries; these results enabled synthesis of a 20-residue peptide that was used to recapitulate the results obtained with MBP-derived peptides. The study shows there is a promiscuous and nucleotide-modulated peptide-binding site in the DEAD Motor domains of SecA.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Receptors and membrane biology
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Cell development, proliferation and death
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
SecApre-protein bindingDEAD MotorBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyReceptors and Membrane BiologyComputational BiologyImmunologyNeuroscienceCell Development, Proliferation and DeathMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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