Dataset for: Effect of diet-induced weight loss on Angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans

Angiopoietin-like protein 4 (ANGPTL4) plays a role in lipid partitioning by inhibiting lipoprotein lipase (LPL)-dependent plasma clearance of triacylglycerol in adipose tissue (AT). We investigated the effects of diet-induced weight loss on plasma ANGPTL4 concentrations in relation to in vivo AT LPL activity and lipolysis and AT ANGPTL4 release in overweight/obese participants. Sixteen individuals (BMI: 28-35 kg/m2; 10 women) were randomized to a dietary intervention composed of either a low-calorie diet (1250 kcal/d) for 12 weeks (n=9) or a very-low-calorie diet (500 kcal/d) for 5 weeks, followed by a 4-week weight stable period. Before and after the intervention, we measured arteriovenous concentration differences in combination with AT blood flow before and after intake of a high-fat mixed-meal with [U-13C]-palmitate to assess in vivo AT LPL activity and lipolysis. The intervention significantly reduced body weight (-8.6±0.6kg, P<0.001). Plasma ANGPTL4 concentrations were unaffected. Significant postprandial AT ANGPTL4 release into the circulation was observed (P<0.01). No association was observed between plasma ANGPTL4 and in vivo LPL activity. After intervention, fasting and postprandial plasma ANGPTL4 concentrations were positively associated with AT free FA (FFA) and glycerol release, reflecting in vivo AT lipolysis (fasting FFA: P=0.039 and postprandial FFA: P=0.003). In conclusion, plasma ANGPTL4 is unaffected by weight loss and is secreted from human AT after a high-fat meal in overweight/obese participants. Plasma ANGPTL4 concentrations were not related to in vivo AT LPL activity, but were positively associated with in vivo AT lipolysis after weight loss.