Dataset 1. glyco and non-glycopeptide.xlsx (20.25 kB)
Dataset for: Enhanced glycosylation of S-layer protein enables a psychrophilic methanogenic archaeon to adapt to elevated temperatures in abundant substrates
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posted on 2019-10-30, 17:14 authored by Lingyan Li, Mifang Ren, Yueqiang Xu, Wenhao Zhang, Cheng Jin, Xiuzhu DongAdaptation to higher temperatures would increase the environmental competitiveness of psychrophiles. Methanolobus psychrophilus, a cold wetland methanogen, “evolved” as a mesophile growing optimally at 30°C after subculturings, and cells grown with ample substrates exhibited higher integrity. This work investigated N-glycosylation of S-layer proteins, the major archaeal envelope component, with respect to mesophilic adaptation. Lectin affinity enriched a glycoprotein from 30°C and ample substrates-grown cells, which was identified as S-layer protein Mpsy_1486. Four N-glycosylation sites were identified, which exhibited different glycosylation profiles with N94 only found in 30°C-culutred cells. An N-linked glycosylation inhibitor, tunicamycin, reduced glycosylation levels of Mpsy_1486 and growth at 30°C, thus establishing a link between S-layer protein glycosylation and higher temperature adaptation of the psychrophilic archaeon M. psychrophilus.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Cell development, proliferation and death
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Receptors and membrane biology
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
Psychrophilic archaeaS-layer proteinglycosylationsubstrate contentscell integritymesophilic adaptationBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyCell Development, Proliferation and DeathComputational BiologyImmunologyNeuroscienceReceptors and Membrane BiologyMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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