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Dataset for: Karyopherin MoKap119-mediated nuclear import of cyclin dependent kinase regulator MoCks1 is essential for Magnaporthe oryzae pathogenicity

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posted on 2019-09-05, 18:54 authored by Shulin Zhang, Chaoxiang Lin, Tian Zhou, Lianhui Zhang, Yizhen Deng
Nuclear import of proteins relies on nuclear import receptors called importins/karyopherins (Kaps), whose functions were reported in yeasts, fungi, plants and animal cells, including cell cycle control, morphogenesis, stress sensing/response, and also fungal pathogenecity. However, limited is known about the physiological function and regulatory mechanism of protein import in the rice-blast fungus Magnaporthe oryzae. Here, we identified an ortholog of β-importin in M. oryzae encoded by an ortholog of KAP119 gene. Functional characterization of this gene via reverse genetics revealed that it is required for vegetative growth, conidiation, melanin pigmentation and pathogenicity of M. oryzae. The mokap119 mutant was also defective in formation of appressorium-like structure from hyphal tips. By affinity assay and LC-MS/MS, we identified potential MoKap119-interacting proteins, and we further verified that MoKap119 interacts with the cyclin dependent kinase subunit MoCks1 and mediates its nuclear import. Transcriptional profiling indicated that MoKap119 may regulate transcription of infection-related genes via MoCks1-regulation of MoSom1. Overall, our findings provide a novel insight into the regulatory mechanism of M. oryzae pathogenesis likely by MoKap119-mediated nuclear import of the cyclin dependent kinase subunit MoCks1.

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