Dataset for: Structural and genetic analysis of START superfamily protein MSMEG_0129 from Mycobacterium smegmatis
datasetposted on 09.04.2018 by Shuping Zheng, Ying Zhou, Joy Fleming, Yafeng Zhou, Mengting Zhang, Shiliang Li, Honglin Li, Bingqi Sun, Wei Liu, Lijun Bi
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Mycobacterium tuberculosis, a notorious pathogen, still threatens human health. Rv0164, an antigen of both T- and B-cells conserved across the mycobacteria, and MSMEG_0129, its close homolog in Mycobacterium smegmatis, are predicted members of the START domain superfamily, but their molecular function is unknown. Here, gene knockout studies demonstrated MSMEG_0129 is essential for bacterial growth, suggesting Rv0164 may be a potential drug target. The MSMEG_0129 crystal structure determined at 1.95 Å reveals a fold similar to that in polyketide aromatase/cyclases ZhuI and TcmN from Streptomyces sp.. Structural comparisons and docking simulations, however, infer that MSMEG_0129 and Rv0164 are unlikely to catalyze polyketide aromatization/cyclization, but probably play an irreplaceable role, for example in lipid transfer, in cell envelope synthesis during mycobacterial growth.