ASM1_FEBS_Lett_SI_26_Nov_2019_accepted.docx (4.2 MB)

Dataset for: Bacteriocin ASM1 is an O/S-diglycosylated, plasmid-encoded homologue of glycocin F

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posted on 2019-12-12, 11:51 authored by Patrick Main, Tomomi Hata, Trevor Loo, Petr Man, Petr Novák, Vladimir Havlicek, Gillian Norris, Mark Langton Patchett
Lactobacillus plantarum A-1 secretes bacteriocin ASM1, with a calculated monoisotopic mass of 4642.8454 Da (based on the 43 amino acid sequence) and a measured mass of 5044.9727 Da. Here we show that ASM1 is a glycocin (glycosylated bacteriocin) with O- and S-linked N-acetylglucosamine moieties identical to glycocin F (GccF), and that the roles of specific structural features of ASM1 are very similar to GccF. Analysis of Lb. plantarum A-1 genomic DNA sequences showed that asm gene organisation (asmH←→asmABCDE→F) is identical to the gcc gene cluster, and that the asm genes reside on the novobiocin-curable 11,905-bp plasmid pA1_ASM1. Amino acid sequence identity averages ~69% across all Asm and Gcc proteins, and is highest for the identical ASM1/GccF peptide scaffold residues 1-30.