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Dataset for: Evaluation of metastatic potential of malignant cells by image processing of digital holographic microscopy data
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posted on 2017-09-02, 05:52 authored by Violeta Liuba Calin, Mona Mihailescu, Eugen I Scarlat, Alexandra Valentina Baluta, Daniel Calin, Eugenia Kovacs, Tudor Savopol, Mihaela Georgeta MoisescuCell refractive index (RI) was proposed as a putative cancer biomarker of great potential, being correlated with cell content and morphology, cell division rate and membrane permeability. We used Digital Holographic Microscopy (DHM) to compare RI and dry mass density of two B16 murine melanoma sublines of different metastatic potential. Using statistical methods, the phase shifts distribution within the reconstructed quantitative phase images (QPIs) was analyzed by the method of bimodality coefficients. The observed correlation of RI and bimodality profile with the cells metastatic potential was validated by real time impedance based-assay and clonogenic tests. We suggest RI and QPIs histograms bimodality analysis to be developed as optical biomarkers useful in label-free detection and quantitative evaluation of cell metastatic potential.
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Categories
- Regenerative medicine (incl. stem cells)
- Synthetic biology
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolution of developmental systems
- Structural biology (incl. macromolecular modelling)
- Proteomics and intermolecular interactions (excl. medical proteomics)
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Bioinformatics and computational biology not elsewhere classified
- Biostatistics
- Computational methods in fluid flow, heat and mass transfer (incl. computational fluid dynamics)
Keywords
digital holographic microscopybimodality coefficient analysis of quantitative phase imagemurine B16 malignant cellsmetastatic potentialimpedance-based cell proliferation testclonogenic testStem CellsSynthetic BiologyMolecular BiologyDevelopmental BiologyStructural BiologyProteomics and Intermolecular Interactions (excl. Medical Proteomics)Evolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyBioinformaticsBiostatisticsHeat and Mass Transfer Operations
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