ETCJ-Oct-18-00586_Supplemental Data.xlsx (232.33 kB)

Dataset for: Factors affecting the growth of Pseudokirchneriella subcapitata in single-species tests: lessons for the experimental design and the reproducibility of a multi-trophic laboratory microcosm

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posted on 2022-01-20, 14:14 authored by Verena Riedl, Annika Agatz, Rachel Benstead, Roman Ashauer
The need for an integrated risk assessment at an ecologically relevant scale (e.g. at the population/community levels) has been acknowledged. Multi-species systems with increased ecological complexity, however, are difficult if not impossible to reproduce. The laboratory scale microcosm TriCosm (Pseudokirchneriella subcapitata, Ceriodaphnia dubia, Hydra viridissima) of intermediate complexity was developed for the reproducible assessment of chemical effects at the population/community levels. The system dynamics were repeatable in the short term but inter-experimental variation of algal dynamics in the long term triggered knock on effects on grazer and predator populations. We present 20 experiments to assess the effects of twelve factors (test medium, vessel type/condition, shaking speed, light intensity/regime, inoculation density, medium preparation components, metal concentration/composition, buffering salt type/concentration) on algal growth in the TriCosm enclosure. Growth rates varied between ≤ 0 and 1.40 (±0.21) and generally were greatest with increased shaking speed, light exposure, medium buffer or aeration time. Treatments conducted in dishes with aseptically prepared, scarcely buffered and/or hardly aerated medium resulted in low growth rates. We found that inter-experimental variation of algal dynamics in the TriCosm was caused by a modification of medium preparation with the aim of reducing microbial contamination, i.e. the omission of medium aeration. Our findings highlight that consistency in experimental procedures and in-depth understanding of system components are indispensable to achieve repeatability.