Ni = Nickel NA = not applicable HC5 = hazardous concentration affecting 5% of the species in a community HC50 = hazardous concentration affecting 50% of the species in a community Day after start experiment Treatment Replicate Pseudanabaenoideae (cyano ) Anabaena (cyano ) Aphanothece (cyano) Aphanocapsa (cyano) Woronichinia (cyano) Microcystisch (cyano) Single cell Diatoms Diatom colony Tetra_dron minimun Tetra_dron triangulare Monoraphidium 1 Monorahidium 2 Scenedesmus 1 Scenedesmus 2 Desmodesmus Cryptomonas Chlamydomonas Rodomonas Gonium Trachelomonas Chrysococcus Radiococcus Staurodesmus Dinobryon sociale Cosmarium Coelastrum Staurastrum Oocystis Carteria Ankistrodesmus Haematococcus Westella botryoides Mougeotia Chlorococcales Euglena (Protozoa) Euglenida 1 Euglenida 2 Chroococcus Oscillatoria 1 Oscillatoria 2 Pteromonas Unknown colony Green algae cology Pleurotaenium Uroglena Tetraspora Chlamydocapsa Lepocinclis Peridinium (dinofl) Merismopedia (cyano) Kirchneriella number of days NA NA number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L number of organisms/L -1 Control a 194925.034 126127.9632 3822.05949 26754.41643 0 0 68797.07082 3822.05949 3822.05949 0 3822.05949 3822.05949 0 3822.05949 0 7644.11898 7644.11898 0 0 0 3822.05949 0 0 0 11466.17847 0 0 0 0 0 0 3822.05949 0 30576.47592 0 0 0 0 7644.11898 0 0 11466.17847 0 0 0 0 0 0 0 0 0 -1 Control b 193014.0042 22932.35694 0 0 0 0 72619.13031 0 0 0 1911.029745 0 0 0 0 1911.029745 3822.05949 0 0 1911.029745 7644.11898 0 7644.11898 0 13377.20821 0 0 1911.029745 0 0 0 1911.029745 0 66886.04107 0 0 7644.11898 1911.029745 13377.20821 0 7644.11898 21021.32719 0 0 0 0 0 0 0 0 0 -1 Control c 131861.0524 85996.33852 3822.05949 11466.17847 0 0 45864.71388 3822.05949 1911.029745 1911.029745 1911.029745 0 1911.029745 1911.029745 0 11466.17847 1911.029745 0 0 0 7644.11898 1911.029745 1911.029745 0 15288.23796 0 7644.11898 0 0 0 0 1911.029745 0 13377.20821 0 0 1911.029745 1911.029745 0 0 0 9555.148725 0 0 0 0 0 0 0 0 0 -1 Control d 103195.6062 0 0 15288.23796 0 0 22932.35694 11466.17847 0 0 0 0 0 7644.11898 0 7644.11898 3822.05949 0 0 0 0 0 3822.05949 0 22932.35694 0 0 3822.05949 0 0 0 0 0 7644.11898 0 0 34398.53541 7644.11898 3822.05949 0 3822.05949 19110.29745 0 0 0 0 0 0 0 0 0 -1 Ni HC5 a 108928.6955 160526.4986 0 3822.05949 0 0 42042.65439 5733.089235 0 0 0 0 1911.029745 0 0 15288.23796 0 0 1911.029745 0 11466.17847 0 0 0 40131.62464 0 1911.029745 0 0 0 0 1911.029745 0 7644.11898 1911.029745 1911.029745 0 1911.029745 1911.029745 0 0 1911.029745 0 0 0 0 0 0 0 0 0 -1 Ni HC5 b 97462.51699 19110.29745 0 17199.2677 0 0 32487.50566 15288.23796 0 0 0 3822.05949 0 36309.56515 3822.05949 3822.05949 1911.029745 0 0 3822.05949 0 0 11466.17847 0 108928.6955 0 3822.05949 0 0 3822.05949 0 7644.11898 0 22932.35694 3822.05949 3822.05949 0 1911.029745 3822.05949 0 0 7644.11898 0 0 0 0 0 0 1911.029745 0 0 -1 Ni HC5 c 103195.6062 0 0 15288.23796 0 0 22932.35694 11466.17847 0 0 0 0 0 7644.11898 0 7644.11898 3822.05949 0 0 0 0 0 3822.05949 0 22932.35694 0 0 3822.05949 0 0 0 0 0 7644.11898 0 0 34398.53541 7644.11898 3822.05949 0 3822.05949 19110.29745 0 0 0 0 0 0 0 0 0 -1 Ni HC50 a 55419.8626 9555.148725 0 7644.11898 0 0 30576.47592 11466.17847 0 0 0 3822.05949 0 15288.23796 0 7644.11898 11466.17847 0 0 0 3822.05949 0 21021.32719 0 85996.33852 0 0 3822.05949 0 0 1911.029745 0 0 11466.17847 0 11466.17847 11466.17847 0 1911.029745 0 3822.05949 3822.05949 0 0 0 0 0 0 0 0 0 -1 Ni HC50 b 194925.034 126127.9632 3822.05949 26754.41643 0 0 68797.07082 3822.05949 3822.05949 0 3822.05949 3822.05949 0 3822.05949 0 7644.11898 7644.11898 0 0 0 3822.05949 0 0 0 11466.17847 0 0 0 0 0 0 3822.05949 0 30576.47592 0 0 0 0 7644.11898 0 0 11466.17847 0 0 0 0 0 0 0 0 0 -1 Ni HC50 c 84085.30878 158615.4688 3822.05949 66886.04107 3822.05949 0 19110.29745 0 0 0 0 0 0 0 0 0 0 0 0 0 3822.05949 0 0 0 36309.56515 0 0 0 0 0 0 0 0 26754.41643 0 22932.35694 0 3822.05949 17199.2677 0 0 7644.11898 0 0 0 0 0 0 0 0 0 14 Control a 85996.33852 3822.05949 0 47775.74362 0 0 3822.05949 0 0 0 1911.029745 0 0 0 0 0 3822.05949 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3822.05949 22932.35694 0 0 11466.17847 3822.05949 110839.7252 0 0 1911.029745 0 0 0 0 0 0 0 0 0 14 Control b 11466.17847 3822.05949 9555.148725 36309.56515 0 0 9555.148725 1911.029745 0 0 0 0 0 0 0 1911.029745 0 0 0 1911.029745 1911.029745 0 0 0 0 0 0 0 0 1911.029745 0 0 1911.029745 166259.5878 0 5733.089235 38220.5949 0 26754.41643 0 0 21021.32719 0 0 0 0 0 0 0 0 0 14 Control c 42042.65439 21021.32719 0 32487.50566 5733.089235 0 49686.77337 0 0 0 0 3822.05949 0 1911.029745 0 0 3822.05949 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 3822.05949 0 0 177725.7663 1911.029745 9555.148725 11466.17847 15288.23796 162437.5283 0 0 32487.50566 0 0 0 0 0 0 0 0 0 14 Control d 36309.56515 45864.71388 1911.029745 28665.44617 1911.029745 1911.029745 26754.41643 0 0 0 0 0 0 0 0 5733.089235 1911.029745 0 0 0 5733.089235 5733.089235 0 0 0 0 0 0 0 0 0 3822.05949 0 21021.32719 0 9555.148725 0 22932.35694 55419.8626 1911.029745 0 57330.89235 0 0 0 0 0 0 0 0 0 14 Ni HC5 a 26754.41643 0 1911.029745 5733.089235 0 0 7644.11898 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 55419.8626 0 15288.23796 1911.029745 15288.23796 15288.23796 0 0 0 0 0 0 0 1911.029745 0 0 0 0 14 Ni HC5 b 17199.2677 0 9555.148725 55419.8626 1911.029745 0 9555.148725 0 0 0 0 0 0 0 0 3822.05949 1911.029745 0 0 1911.029745 3822.05949 0 0 0 0 0 0 0 0 0 0 0 0 143327.2309 0 0 28665.44617 11466.17847 49686.77337 0 0 13377.20821 0 0 0 0 0 0 0 0 0 14 Ni HC5 c 11466.17847 162437.5283 0 63063.98158 0 0 7644.11898 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 3822.05949 0 0 0 13377.20821 0 0 0 0 0 0 0 0 22932.35694 0 3822.05949 3822.05949 21021.32719 11466.17847 0 0 3822.05949 0 0 0 0 0 0 0 0 0 14 Ni HC50 a 13377.20821 15288.23796 5733.089235 64975.01133 0 0 80263.24929 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 1911.029745 0 0 5733.089235 22932.35694 0 5733.089235 1911.029745 0 3822.05949 0 0 9555.148725 0 0 1911.029745 0 0 0 0 0 0 14 Ni HC50 b 7644.11898 24843.38668 24843.38668 38220.5949 0 0 68797.07082 0 0 0 1911.029745 0 0 0 0 5733.089235 9555.148725 0 1911.029745 0 0 0 3822.05949 0 21021.32719 0 1911.029745 1911.029745 0 3822.05949 15288.23796 0 3822.05949 22932.35694 0 26754.41643 7644.11898 0 5733.089235 3822.05949 0 0 0 0 0 0 0 0 0 0 0 14 Ni HC50 c 40131.62464 19110.29745 1911.029745 42042.65439 0 0 72619.13031 11466.17847 1911.029745 0 0 7644.11898 0 5733.089235 1911.029745 21021.32719 9555.148725 0 3822.05949 7644.11898 17199.2677 0 72619.13031 0 47775.74362 0 1911.029745 0 0 7644.11898 26754.41643 13377.20821 9555.148725 24843.38668 0 13377.20821 1911.029745 5733.089235 3822.05949 0 0 0 0 0 0 0 0 0 0 0 0 28 Control a 0 0 1911.029745 32487.50566 0 1911.029745 7644.11898 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 1911.029745 0 0 0 0 21021.32719 3822.05949 0 9555.148725 3822.05949 0 269455.194 0 0 3822.05949 0 0 0 0 0 0 0 0 0 28 Control b 0 0 0 15288.23796 1911.029745 0 9555.148725 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 0 1911.029745 13377.20821 0 36309.56515 30576.47592 0 294298.5807 0 0 5733.089235 0 0 0 0 0 0 0 0 0 28 Control c 0 0 0 5733.089235 0 1911.029745 1911.029745 3822.05949 0 0 0 0 0 0 0 1911.029745 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 1911.029745 3822.05949 22932.35694 1911.029745 1911.029745 26754.41643 1911.029745 324875.0566 0 0 7644.11898 0 0 0 0 0 0 0 0 0 28 Control d 0 1911.029745 0 11466.17847 0 0 1911.029745 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 1911.029745 0 85996.33852 7644.11898 1911.029745 389850.068 0 0 9555.148725 0 0 0 0 0 0 0 0 0 28 Ni HC5 a 1911.029745 5733.089235 1911.029745 3822.05949 0 5733.089235 5733.089235 0 0 0 0 0 0 0 3822.05949 1911.029745 1911.029745 0 0 0 3822.05949 1911.029745 0 0 1911.029745 3822.05949 0 0 0 0 0 0 0 210213.2719 0 0 17199.2677 7644.11898 63063.98158 1911.029745 0 34398.53541 0 0 0 0 0 0 0 1911.029745 0 28 Ni HC5 b 1911.029745 0 0 3822.05949 0 0 5733.089235 1911.029745 0 0 1911.029745 0 0 0 0 1911.029745 1911.029745 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1911.029745 11466.17847 0 1911.029745 24843.38668 1911.029745 45864.71388 0 1911.029745 1911.029745 0 0 0 0 0 0 0 0 0 28 Ni HC5 c 1911.029745 28665.44617 0 32487.50566 0 0 7644.11898 1911.029745 0 0 0 0 0 0 0 11466.17847 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13377.20821 9555.148725 0 11466.17847 7644.11898 0 124216.9334 0 0 0 1911.029745 0 0 0 1911.029745 0 0 0 0 28 Ni HC50 a 0 0 1911.029745 32487.50566 1911.029745 0 13377.20821 0 0 0 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 1911.029745 1911.029745 0 0 3822.05949 0 1911.029745 40131.62464 0 34398.53541 0 0 1911.029745 7644.11898 0 0 0 0 0 0 0 0 28 Ni HC50 b 5733.089235 0 0 28665.44617 0 0 38220.5949 5733.089235 0 0 1911.029745 1911.029745 0 0 0 0 0 0 0 7644.11898 0 0 3822.05949 0 17199.2677 0 1911.029745 1911.029745 0 0 0 0 47775.74362 0 3822.05949 7644.11898 72619.13031 0 9555.148725 0 1911.029745 5733.089235 7644.11898 0 0 0 1911.029745 0 0 0 0 28 Ni HC50 c 5733.089235 0 7644.11898 24843.38668 0 0 64975.01133 1911.029745 0 0 0 0 0 0 1911.029745 7644.11898 1911.029745 0 1911.029745 0 1911.029745 0 11466.17847 0 3822.05949 0 7644.11898 7644.11898 0 0 1911.029745 0 17199.2677 5733.089235 0 1911.029745 7644.11898 0 3822.05949 0 0 0 5733.089235 0 0 0 0 0 0 0 0 42 Control a 0 0 0 0 0 0 1911.029745 0 0 0 1911.029745 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 28665.44617 9555.148725 0 22932.35694 5733.089235 0 110839.7252 0 1911.029745 0 0 0 0 0 0 0 0 0 0 42 Control b 1911.029745 1911.029745 0 3822.05949 0 0 7644.11898 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 0 0 1911.029745 1911.029745 0 0 0 0 0 0 19110.29745 51597.80311 0 15288.23796 17199.2677 0 236967.6884 0 1911.029745 13377.20821 0 0 0 0 0 0 0 0 0 42 Control c 0 0 0 3822.05949 0 0 1911.029745 0 0 0 1911.029745 0 1911.029745 0 0 0 1911.029745 0 0 1911.029745 0 0 0 0 0 5733.089235 0 0 0 0 1911.029745 0 21021.32719 36309.56515 1911.029745 34398.53541 11466.17847 0 303853.7295 0 0 5733.089235 0 0 0 0 0 0 0 0 0 42 Control d 0 0 3822.05949 5733.089235 0 3822.05949 1911.029745 0 0 0 3822.05949 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 0 0 5733.089235 38220.5949 0 15288.23796 5733.089235 0 269455.194 3822.05949 7644.11898 9555.148725 0 0 0 0 0 0 0 0 0 42 Ni HC5 a 1911.029745 0 0 15288.23796 0 7644.11898 5733.089235 0 1911.029745 0 1911.029745 1911.029745 0 0 0 0 1911.029745 11466.17847 1911.029745 3822.05949 1911.029745 0 0 0 0 5733.089235 0 0 0 0 0 0 7644.11898 154793.4093 0 9555.148725 95551.48725 9555.148725 122305.9037 0 3822.05949 15288.23796 0 0 0 0 0 0 0 0 0 42 Ni HC5 b 0 0 0 1911.029745 0 0 9555.148725 0 0 0 0 0 0 0 0 7644.11898 7644.11898 0 0 3822.05949 1911.029745 0 0 0 0 0 0 0 0 0 0 0 0 17199.2677 5733.089235 26754.41643 17199.2677 3822.05949 131861.0524 0 0 5733.089235 0 0 0 0 0 0 0 0 0 42 Ni HC5 c 1911.029745 7644.11898 0 7644.11898 0 0 7644.11898 0 1911.029745 0 1911.029745 0 0 0 0 1911.029745 0 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 0 42042.65439 13377.20821 3822.05949 87907.36827 5733.089235 1911.029745 185369.8853 1911.029745 0 0 5733.089235 0 0 0 1911.029745 0 0 0 0 42 Ni HC50 a 0 0 0 9555.148725 0 0 5733.089235 0 0 0 5733.089235 0 0 0 0 1911.029745 3822.05949 0 0 0 7644.11898 0 0 0 0 0 0 0 0 0 0 0 5733.089235 3822.05949 1911.029745 11466.17847 3822.05949 0 21021.32719 0 5733.089235 0 0 1911.029745 0 0 0 0 0 0 0 42 Ni HC50 b 0 0 34398.53541 17199.2677 0 0 11466.17847 0 0 0 3822.05949 0 1911.029745 0 0 5733.089235 1911.029745 3822.05949 0 1911.029745 5733.089235 0 0 0 1911.029745 0 0 3822.05949 0 0 0 0 5733.089235 9555.148725 5733.089235 40131.62464 36309.56515 0 1911.029745 0 0 0 3822.05949 0 0 0 0 0 0 0 0 42 Ni HC50 c 0 0 0 13377.20821 0 0 30576.47592 0 0 0 1911.029745 0 1911.029745 3822.05949 0 5733.089235 0 0 0 0 3822.05949 1911.029745 1911.029745 0 0 0 5733.089235 1911.029745 0 0 1911.029745 1911.029745 3822.05949 3822.05949 0 1911.029745 19110.29745 1911.029745 1911.029745 0 0 0 1911.029745 0 0 0 0 0 0 0 0 56 Control a 1911.029745 0 0 11466.17847 0 0 11466.17847 0 0 0 0 0 0 0 0 0 1911.029745 1911.029745 0 0 0 0 0 0 0 0 0 1911.029745 0 0 0 0 208302.2422 22932.35694 0 32487.50566 34398.53541 7644.11898 26754.41643 0 1911.029745 0 0 0 0 0 0 0 0 0 0 56 Control b 5733.089235 1911.029745 9555.148725 26754.41643 0 0 9555.148725 0 0 1911.029745 9555.148725 1911.029745 0 0 0 1911.029745 5733.089235 0 0 0 7644.11898 0 0 0 0 1911.029745 0 0 0 0 0 0 89818.39801 91729.42776 3822.05949 55419.8626 0 5733.089235 189191.9448 0 0 15288.23796 0 0 0 0 0 0 0 0 0 56 Control c 0 0 22932.35694 11466.17847 0 1911.029745 0 1911.029745 0 0 1911.029745 0 0 0 0 1911.029745 0 0 0 5733.089235 1911.029745 0 0 0 0 0 0 1911.029745 0 0 0 0 40131.62464 1911.029745 1911.029745 3822.05949 0 7644.11898 298120.6402 0 5733.089235 11466.17847 0 0 0 0 0 0 0 0 0 56 Control d 0 0 5733.089235 7644.11898 0 0 0 0 1911.029745 0 0 0 1911.029745 0 3822.05949 1911.029745 0 0 0 1911.029745 0 0 0 0 0 0 0 0 0 0 1911.029745 0 24843.38668 173903.7068 3822.05949 15288.23796 13377.20821 9555.148725 366917.711 0 0 13377.20821 0 0 0 0 0 0 0 0 0 56 Ni HC5 a 1911.029745 0 1911.029745 3822.05949 0 0 1911.029745 0 0 0 3822.05949 0 0 0 0 0 0 3822.05949 0 0 0 0 0 0 0 1911.029745 0 1911.029745 0 0 0 0 7644.11898 64975.01133 0 227412.5397 9555.148725 3822.05949 120394.8739 0 1911.029745 7644.11898 0 1911.029745 0 0 0 0 0 0 0 56 Ni HC5 b 0 0 70708.10056 3822.05949 0 7644.11898 11466.17847 0 0 0 0 0 3822.05949 0 0 3822.05949 1911.029745 5733.089235 0 0 5733.089235 1911.029745 0 0 0 0 1911.029745 3822.05949 0 0 0 0 9555.148725 40131.62464 5733.089235 21021.32719 3822.05949 3822.05949 221679.4504 1911.029745 1911.029745 11466.17847 0 0 0 0 0 0 0 0 0 56 Ni HC5 c 0 0 1911.029745 7644.11898 0 0 5733.089235 0 0 0 0 0 0 0 0 0 3822.05949 0 0 0 1911.029745 0 0 0 1911.029745 0 0 0 0 0 0 0 305764.7592 3822.05949 0 110839.7252 11466.17847 0 51597.80311 0 0 0 0 0 0 0 0 0 0 0 0 56 Ni HC50 a 0 0 1911.029745 22932.35694 0 0 15288.23796 0 0 0 3822.05949 0 0 0 0 0 3822.05949 5733.089235 0 1911.029745 5733.089235 0 0 0 1911.029745 0 0 0 0 0 0 0 82174.27903 9555.148725 5733.089235 30576.47592 22932.35694 0 15288.23796 1911.029745 0 0 63063.98158 0 0 0 0 0 0 0 0 56 Ni HC50 b 0 0 0 13377.20821 0 0 11466.17847 0 0 0 0 0 0 0 0 3822.05949 0 1911.029745 0 0 19110.29745 0 3822.05949 0 9555.148725 0 0 0 0 0 0 0 28665.44617 13377.20821 11466.17847 17199.2677 24843.38668 3822.05949 9555.148725 0 5733.089235 3822.05949 49686.77337 0 0 0 0 0 0 0 0 56 Ni HC50 c 0 0 0 13377.20821 0 0 26754.41643 1911.029745 0 0 0 0 0 0 0 5733.089235 1911.029745 1911.029745 0 1911.029745 3822.05949 0 1911.029745 0 0 0 0 1911.029745 0 0 0 11466.17847 24843.38668 9555.148725 0 3822.05949 63063.98158 0 3822.05949 0 0 0 0 0 0 0 0 0 0 0 0