FabT_validation report.pdf (498.86 kB)
Dataset for: Structural insights into repression of Pneumococcal fatty acid synthesis pathway via repressor FabT and co-repressor acyl-ACP
dataset
posted on 2019-07-31, 12:19 authored by Gang Zuo, Zhi-Peng Chen, Yong-Liang Jiang, Zhongliang Zhu, Chengtao Ding, Zhiyong Zhang, Yuxing Chen, Cong-Zhao Zhou, Qiong LiStreptococcus pneumoniae fatty acid synthesis pathway is globally controlled at the transcriptional level by repressor FabT and co-repressor acyl-ACP, the intermediate of phospholipid synthesis. Here we report the crystal structure of FabT complexed with a 23-bp dsDNA, indicating that FabT is a weak repressor of low DNA-binding affinity in the absence of acyl-ACP. Modification of ACP with a long-chain fatty acid is necessary for the formation of a stable complex with FabT, mimicking in vitro by cross-linking, which would significantly elevate the DNA-binding affinity of FabT. Altogether, we propose a putative working model of gene repression under the double controls of FabT and acyl-ACP, elucidating a distinct repression network for Pneumococcus to precisely coordinate fatty acid synthesis.
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Categories
- Animal physiology - biophysics
- Human biophysics
- Synthetic biology
- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Virology
- Cell development, proliferation and death
- Bioinformatics and computational biology not elsewhere classified
- Immunology not elsewhere classified
- Neurosciences not elsewhere classified
- Receptors and membrane biology
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Structural biology (incl. macromolecular modelling)
Keywords
fatty acid synthesistranscription repressorco-repressorrepressionBiophysicsSynthetic BiologyBiochemistryPlant BiologyVirologyCell Development, Proliferation and DeathComputational BiologyImmunologyNeuroscienceReceptors and Membrane BiologyMolecular BiologyEvolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyStructural Biology
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